Coding

Part:BBa_K1935003:Design

Designed by: Emilie Gounin   Group: iGEM16_Bordeaux   (2016-10-13)


White collar-1 (WC-1) - photoreceptor in Neurospora tetrasperma


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2220
    Illegal BglII site found at 2856
    Illegal BamHI site found at 2501
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1783
    Illegal BsaI site found at 2127
    Illegal BsaI site found at 2580
    Illegal SapI.rc site found at 2722
    Illegal SapI.rc site found at 3021


Design Notes

A mutagenesis ha done to abolish an illegal restriction site (EcoR1 : 1949 to 1954 pb, GAATTC => GAATCC)


Source

http://www.uniprot.org/uniprot/F8MUC4

References