Composite

Part:BBa_K1933101:Design

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)


constitutive expression of CBDclos fused to INPNC with 6xHis tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal NgoMIV site found at 465
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon for this part is not in the coding region, but in the suffix region.


Source

  • BBa_K1933004: Promoter and RBS
  • BBa_K1933011: The INPNC surface expression component derives from Pseudomonas Syringae .
    We used a part BBa_K811005 from iGEM12_Penn for construction of BBa_K1933011.
  • BBa_K1321341 from iGEM Imperial 2014: CBDclos derives from Clostridium cellulovorans , and is part of a multi-unit cellulosomes.