Composite
U6 array

Part:BBa_K1925004:Design

Designed by: Zi Yue Wang   Group: iGEM16_Fudan   (2016-10-11)


3xtetO-U6 tandom repeat(U6 array)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 373
    Illegal BamHI site found at 751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use proper restriction enzymes and design proper enzyme site behind and before your gene of interest


Source

Our iGEM team built it

References