Composite

Part:BBa_K1922012:Design

Designed by: WANG HONGNING   Group: iGEM16_Tongji_Shanghai   (2016-10-14)


hsp70-p53-luciferase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 8
    Illegal PstI site found at 359
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 270
    Illegal NheI site found at 2216
    Illegal PstI site found at 8
    Illegal PstI site found at 359
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2537
    Illegal BamHI site found at 415
    Illegal BamHI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 8
    Illegal PstI site found at 359
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 8
    Illegal PstI site found at 359
    Illegal NgoMIV site found at 2571
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 220
    Illegal BsaI.rc site found at 1322
    Illegal SapI site found at 404


Design Notes

It will optimize the efficiency of heat treatment.


Source

It comes from specific genomic sequence of our submitted basic parts.

References