Part:BBa_K1922007:Design

hsp70-p53

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 269
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 414
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 219
  Illegal BsaI.rc site found at 1321
  Illegal BsaI.rc site found at 2512
  Illegal SapI site found at 403
  

Design Notes

This combination can start cell apoptosis by heating,as an optimization of traditional heat treatment.

Source

This plasmid is built based on genomic sequence of HSP and P53 coding sequence.