Part:BBa_K1922006
hTERT-p53
Introduction
Htert-P53 is a combination for tumor cells.The Htert promoter is a telomerase specific promoter,P53 is a common gene surppression gene.loading this plasmid into the mixture of tumor cells and normal cells,only the tumor cells will have a high level expression of P53,therefore managed to reach specificity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 263
Illegal BsaI.rc site found at 1610
Construction
PCR:
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Function
To test if the hTERT promoter works, we construct a plasmid in which the promoter is linked with GFP. By observing the cell in fluorescence microscope and compare GFP expression in both telomerase possitive and negative cells, we could make sure p53 following the promoter can over express in tumor cells specifically. Next, we infected hTERT-p53 into breast cancer cell line hcc 1937 and treated it with 42°C then analyzed the cellular viability.
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