Coding

Part:BBa_K1921018

Designed by: Zhuozhi Chen   Group: iGEM16_TJUSLS_China   (2016-10-14)


LPP-OmpA+linker.a


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

We add a GGGGSGGGGS linker at the C-terminal of Lpp-OmpA in order to minimize its potential influence on our target protein.

Pre-expression

Tjuresults41.jpg

Figure 1.This is the pre-expression using E.coli BL21 at 37 ℃.

Tjuresults43.jpg

Figure 2. This is the pre-expression using E.coli BL21 at 16 ℃.

Tjuresults42.jpg

Figure 3. This is the pre-expression using E.coli BL21 at 25 ℃.


Surface display HPLC results

ProofTJU8.jpg

Figure 4. Relative enzyme activity of engineering bacteria E.coli(BL21)/pET22b(+)LAP when induced at 16℃.

[edit]
Categories
//awards/part_collection/2016
Parameters
None