Coding

Part:BBa_K192100:Design

Designed by: Anish Kapadia   Group: iGEM09_TorontoMaRSDiscovery   (2010-06-22)


catechol 1 2-dioxygenase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 259
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 259
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 259
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 259
    Illegal NgoMIV site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was retrieved from NCBI nucleotide database. We needed to account for a restriction site located mid-sequence.


Source

DNA sequence is from the genomic sequence of the pseudomonas putida kt2440 strain.

Direct Submission to The National Genomic Institute.

Authors

Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,

          Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
          Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
          Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
          Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
          Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
          Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
          Duesterhoft,A., Tummler,B. and Fraser,C.


References