Coding
Part:BBa_K192100:Design
Designed by: Anish Kapadia Group: iGEM09_TorontoMaRSDiscovery (2010-06-22)
catechol 1 2-dioxygenase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 259
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 259
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 259
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 259
Illegal NgoMIV site found at 283 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was retrieved from NCBI nucleotide database. We needed to account for a restriction site located mid-sequence.
Source
DNA sequence is from the genomic sequence of the pseudomonas putida kt2440 strain.
Direct Submission to The National Genomic Institute.
Authors
Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,
Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
Duesterhoft,A., Tummler,B. and Fraser,C.