Part:BBa_K1919303:Design

livH

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 631
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 953
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 910
  

Design Notes

The restriction sites are eliminated by mutation.

Source

The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.