Coding
Part:BBa_K1919000:Design
Designed by: Shiji Zhao Group: iGEM16_SCU-China (2016-09-04)
The coding sequence of antimicrobial peptide CecropinXJ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence was optimized for E.coli expression using JCat http://www.jcat.de/. The single stop codon was replaced by double TAA stop codon.
Source
The original sequence of CecropinXJ comes from GenBank NM_001043995.1. The sample used for experiments was artificially synthesized by Tsingke.
References
Xia L, Zhang F, Liu Z, et al. Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli[J]. Experimental & Therapeutic Medicine, 2013, 5(6):1745-1751.