Composite

Part:BBa_K1918107:Design

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)


CMV-Kozak-IntC:EBFP2C(155-238)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1136
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1136
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1136
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


Design Notes

AIM: IntC:EBFP2C(155-238) could be expressed in cell all time; ELEMENTS: IntC, EBFP2C(155-238), CMV

Source

PCR from the existing plasmid in xie lab

References