Composite

Part:BBa_K1918103:Design

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)


TRE-Kozak-EBFP2N(1-154):IntN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AIM: the expression of EBFP2N(1-154):IntN could be induced by Dox; ELEMENTS: EBFP2N(1-154), IntN, Dox-induced promoter TRE

Source

PCR from the existing plasmid in xie lab

References