Composite

Part:BBa_K1916100:Design

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-09)


Ara-Inducible NpF2164g5 Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal SpeI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
    Illegal NgoMIV site found at 1182
    Illegal AgeI site found at 1272
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

Protein from cyanobacteria genome (sequence provided with assistance of Nathan Rockwell and Clark Lagarias of the Lagarias Lab, UC Davis). pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

References