Part:BBa_K1913023:Design

According to some previous iGEM projects (UNITN-Trento 2013, INSA-Toulouse 2013), the transcription activity of the wild type Fixk2 promoter is so weak that they all added an inverter part to control their target gene expression. Even the original pDusk system in darkness has only 5 times expression levels than in light conditions. Therefore, we decided to enhance the transcriptional activity of the Fixk2 promoter by changing the core element region of the wild type Fixk2 by changing core element into -10 to -35 region of ompC promoter from another two component system (Mizuno T et al., 1986), because we couldn’t guarantee that FixJ boxes could be compatible with the core element of a constitutive promoter.

References

Mizuno T, Mizushima S. Characterization by deletion and localized mutagenesis in vitro of the promoter region of the Escherichia coli ompC gene and importance of the upstream DNA domain in positive regulation by the OmpR protein[J]. Journal of bacteriology, 1986, 168(1): 86-95.