Coding
Part:BBa_K1913000:Design
Designed by: Lisa Röttjers Group: iGEM16_Wageningen_UR (2016-09-22)
chiA for Varroa destructor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was optimized using the IDT codon optimization tool. Multiple restriction sites were removed; BsaI at position 195, PstI at position 644, PstI at position 905 and EcoRI at position 943. Additionally, several antisense stop codons were introduced to prevent antisense transcription.
Source
The source of this part is chiA from Serratia marcescens GEI; accession GQ855217.