Coding

Part:BBa_K1913000:Design

Designed by: Lisa Röttjers   Group: iGEM16_Wageningen_UR   (2016-09-22)


chiA for Varroa destructor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized using the IDT codon optimization tool. Multiple restriction sites were removed; BsaI at position 195, PstI at position 644, PstI at position 905 and EcoRI at position 943. Additionally, several antisense stop codons were introduced to prevent antisense transcription.


Source

The source of this part is chiA from Serratia marcescens GEI; accession GQ855217.

References