Part:BBa_K191007:Design

Trp promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• Notice: 2 SpeI enzyme restriction sites on Trp promoter sequence.

• Trp promoter sequence is ordered as primer and added to part BBa_K191004 and BBa_K191005 by PCR.

Source

Sequence of Trp operon was found at : http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00067