Coding
LovTAP

Part:BBa_K191006:Design

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-16)

LOVTAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535


Design Notes

  • Notice: LOVTAP sequence contains 2 PstI restriction sites.
  • LovTAP was obtained by PCR with primers containing iGEM prefix and suffix ( with enzyme retriction sites of EcoRI, SpeI, XbaI, PstI ).
  • Digestion with E and S.
  • Ligated into empty plasmid psB1A3.

Source

Plasmid with LovTAP gene was kindly provided by the authors of : Light-activated DNA binding in a designed allosteric protein; Strickland et al., Proceedings of the National Academy of Sciences (2008) vol. 105 (31) pp. 10709

References

Light-activated DNA binding in a designed allosteric protein; Strickland et al., Proceedings of the National Academy of Sciences (2008) vol. 105 (31) pp. 10709