Protein_Domain
LtrA

Part:BBa_K1906003

Designed by: Yuwei Han   Group: iGEM16_XJTLU-CHINA   (2016-09-11)


Intron encoded protein LtrA

LtrA is a intron-encoded protein (IEP) from Lactococcus lactis. LtrA is a multifunctional protein that promotes group II intron splicing and mobility.

Usage and Biology

This enzyme is a maturase that catalyzes the formation of mature form of introns, a endonuclease once bind with mature RNAs and a reverse transcriptase that can drive the formation of cDNA strand of RNA it carries.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1093
    Illegal BamHI site found at 1469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 291


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Categories
Parameters
None