RNA

Part:BBa_K1906002

Designed by: Yuwei Han   Group: iGEM16_XJTLU-CHINA   (2016-09-11)


LtrB Group II intron tail part

A Group II intron is a retrohoming element that can mediate the insertion of itself to a certain location of DNA. The Lactococcus lactis LtrB intron is a group II intron that can target the ligated-exon sequence of the L. lactis ltrB gene. The general structure of wild-type LtrB Group II intron is showed in Figure 1.

Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.


This part is the tail segment of the intron RNA, ranging from the stop codon of LtrA protein to the end of the transcript.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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