Composite

Part:BBa_K1905003:Design

Designed by: Juan Carlos Rueda Silva   Group: iGEM16_TecCEM_HS   (2016-09-13)


Riboswitch to detect E5 mRNA from HPV-16 and express RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 838
    Illegal AgeI site found at 950
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The main design consideration is the proper fold of the mRNA and hybridization with E5 mRNA.


Source

It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of E5 mRNA and code RFP.

References