Coding

Part:BBa_K1903016:Design

Designed by: Rafael Montenegro Marín   Group: iGEM16_TEC-Costa_Rica   (2016-10-17)


dCas9 A coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1052
    Illegal BglII site found at 2822
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3536
    Illegal SapI.rc site found at 1165


Design Notes

This CDS was assembled with Golden Gate using four gBlocks.


Source

The source for the Cas9 sequence was UNIPROT accession: Q99ZW2. We optimized the sequence for E. coli and changed the amino acids at positions D10A and H840A for making the protein catalytically inactive.

References