Device
Part:BBa_K1903015:Design
Designed by: Sofía Vieto Fonseca Group: iGEM16_TEC-Costa_Rica (2016-10-14)
PA1-RBS + GFP-linker-Quencher
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Design Notes
This is a device created with the BioBricks GFP-TEVLinker-DarkQuencher BBa_K1903004 and the Anderson promoter BBa_J23101 assembled to a RBS Elowitz BBa_B0034 .
We assembled this device and verified it in an agarose gel electrophoresis: