Device

Part:BBa_K1903013:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


Plac-RBS-Split TEV-IntN DnaE + PA1-RBS + Split TEV-IntC DnaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1044
    Illegal NheI site found at 1067
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544
    Illegal SapI.rc site found at 1209
    Illegal SapI.rc site found at 1519


Design Notes

For this BioBrick we fused two devices BBa_K1903006 and BBa_K1903008. This is a system that will react Inteins from DnaE to trigger the reconstitution of the TEV, under the presence of IPTG to induce the BBa_K1903006 BioBrick.

Source

References