Generator

Part:BBa_K190074:Design

Designed by: Wilfred Poppinga   Group: iGEM09_Groningen   (2009-10-17)


Gas Vesicle polycistonic gene without repeat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
    Illegal BamHI site found at 3739
    Illegal XhoI site found at 3427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1187
    Illegal AgeI site found at 3706
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2597
    Illegal SapI.rc site found at 3353


Design Notes

Using XhoI and MvaI the repeated sequence was removed and replaced by a synthetic construct containing only one copy.


Source

No part name specified with partinfo tag. designed by iGEM07_Melbourne

References