![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K190038:Design
Designed by: Nienke Kuipers Group: iGEM09_Groningen (2009-09-08)
Arsenic accumulation device (lactose promoter + fMT + GlpF)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 246
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 335
Illegal NgoMIV site found at 719 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon.
Source
The device is composed from BBa_K190032, which contains the lactose promoter (BBa_R0010) together with fMT ( BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter.