Composite

Part:BBa_K190026:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-08-25)


GVP with medium constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
    Illegal BamHI site found at 4182
    Illegal XhoI site found at 3870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 828
    Illegal NgoMIV site found at 1230
    Illegal AgeI site found at 4149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2640
    Illegal SapI.rc site found at 3796


Design Notes

The promoter was ligated into the vector, followed by putting the GVP cluster behind it.


Source

Both subparts and vector were taken from the registry.

References