Regulatory

Part:BBa_K1899004:Design

Designed by: iGEM2016_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-02)


phlFp promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since it is a new promoter, forward and reverse oligos of PphlF with XbaI and PstI sticky ends were designed and ordered, which were subsequently phosphorylated and annealed.



Source

http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

References