Part:BBa_K1899004

phlFp promoter

phlFp - PhlF repressible promoter

Construct for characterization

Fig.1 phlFp with reporter gene.

In order to compare the promoter strength of the phlFp with the other two promoters, tetp and lacp, it was ligated with a GFP reporter gene, BBa_E0240, in BioBrick RFC10 standard.

Results

Experiments on comparing the strength of phlFp, tetp and lacp, were performed. Results indicated that phlFp is the strongest among the three, with 1.96 times and 6 times stronger than that of tetp and lacp, respectively.

Fig 2. Comparison on the strength of phlFp, tetp and lacp. Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

TetR Orthologs Collection

Promoter		CDS
pAmeR	BBa_J428000	AmeR	BBa_J428008
pAmtR	BBa_J428001	AmtR	BBa_J428009
pBetI	BBa_J428053	BetI	BBa_J428010
pBM3R1	BBa_J428054	BM3R1	BBa_J428045
pButR	BBa_J428002	ButR	BBa_J428011
pHapR	BBa_J428003	HapR	BBa_J428012
pHlyllR	BBa_J428004	HlyllR	not added
pIcaRA	BBa_J428042	IcaRA	BBa_J428044
pLitR	not added	LitR	BBa_J428043
pLmrA	not added	LmrA	BBa_J428013

Promoter		CDS
pMcbR	BBa_J428052	McbR	BBa_J428014
pOrf2	not added	Orf2	BBa_J428015
pPhlF	BBa_K1899004	PhlF	BBa_J428016
pPsrA	BBa_J428005	PsrA	BBa_J428017
pQacR	BBa_J428050	QacR	BBa_J428018
pScbR	BBa_J428006	ScbR	BBa_J428019
pSmcR	BBa_J428007	SmcR	not added
pSrpR	BBa_J428049	SrpR	BBa_J428020
pTarA	BBa_J428048	TarA	BBa_J428021

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165527/