Part:BBa_K1897010:Design

Invasin CDS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 2701
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 886
  Illegal AgeI site found at 2395
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Only the coding sequence (CDS) was taken from the genebank sequence.

Source

Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [Genbank M17448.1].

References

Isberg, R. R., & Falkow, S. (1985). A single genetic locus encoded by Yersinia pseudotuberculosis permits invasion of cultured animal cells by Escherichia coli K-12. Nature, 317(6034), 262-264.

Isberg, R. R., & Leong, J. M. (1988). Cultured mammalian cells attach to the invasin protein of Yersinia pseudotuberculosis. Proceedings of the National Academy of Sciences, 85(18), 6682-6686.

McCormick, B. A., Nusrat, A., Parkos, C. A., D'Andrea, L., Hofman, P. M., Carnes, D., Liang, T.W. & Madara, J. L. (1997). Unmasking of intestinal epithelial lateral membrane beta1 integrin consequent to transepithelial neutrophil migration in vitro facilitates inv-mediated invasion by Yersinia pseudotuberculosis. Infection and immunity, 65(4), 1414-1421.