Coding
Part:BBa_K1896008:Design
Designed by: Bob Van Hove, Maarten Van Brempt Group: iGEM16_UGent_Belgium (2016-10-13)
INP_RC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 142
Illegal NgoMIV site found at 1654
Illegal NgoMIV site found at 1798
Illegal NgoMIV site found at 2062
Illegal NgoMIV site found at 2206
Illegal NgoMIV site found at 2254
Illegal NgoMIV site found at 2374
Illegal AgeI site found at 1318 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The repeat domain in this part seems to be impossible to create synthetically so PCR was used instead, with primers binding just outside of the repeat region. The absence of a stop codon allows for seamless assembly of fusion proteins.
Source
References
- Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from Pseudomonas syringae S203. Molecular and General Genetics MGG, 215(1), 165-172.
- Li, L., Gyun Kang, D., & Joon Cha, H. (2004). Functional display of foreign protein on surface of Escherichia coli using N‐terminal domain of ice nucleation protein. Biotechnology and bioengineering, 85(2), 214-221.