Translational_Unit
Gp2
Part:BBa_K1893019:Design
Designed by: Henry Lloyd-Laney, Stefan Grossfurthner Group: iGEM16_Imperial_College (2016-10-14)
T7 phage gene product 2 (Gp2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 118
Design Notes
The gp2 coding sequence was initially obtained by synthesis using the ThermoFisher GeneArt service, based on the T7 phage reference genome provided in the NCBI nucleotide database (NC_001604). The native RBS was replaced with a standard Elowitz RBS (BBa_B0034) and the resulting sequence was flanked with BioBrick prefix and suffix sequences. The modified coding sequence was PCR amplified from the provided company plasmid cloned into our backbone of choice.
Source
Gp2 and an RBS was synthesised as a single gene block using the GeneArt® Gene Synthesis service by Thermo Fisher