Composite
Part:BBa_K1893001:Design
Designed by: Alyssa Henderson, Aditi Satija Group: iGEM16_Imperial_College (2016-10-14)
Las receiver with GFP reporter (LasR+pLas+GFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1719
Design Notes
This part does not include an LVA tag on the LasR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pLas promoter, activating transcription of downstream genes. This part contains everything necessary for 3O-C12-HSL-induced expression of genes inserted downstream of the pLas promoter, which is, in this case, GFPmut3b. GFPmut3b was included in this part so that we could characterize the activation range of the LasR system.
Source
Freemont Lab, Imperial College London