Device

Part:BBa_K189102:Design

Designed by: GuoQiang Chen   Group: iGEM09_Tsinghua   (2009-10-20)


pAC linkage group of GenSniper via Bottom Up approach


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1319
    Illegal XbaI site found at 7320
    Illegal PstI site found at 1616
    Illegal PstI site found at 1816
    Illegal PstI site found at 2027
    Illegal PstI site found at 2121
    Illegal PstI site found at 2589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1319
    Illegal NheI site found at 6479
    Illegal PstI site found at 1616
    Illegal PstI site found at 1816
    Illegal PstI site found at 2027
    Illegal PstI site found at 2121
    Illegal PstI site found at 2589
    Illegal NotI site found at 4876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1319
    Illegal BglII site found at 5032
    Illegal BamHI site found at 2412
    Illegal XhoI site found at 5081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1319
    Illegal XbaI site found at 7320
    Illegal PstI site found at 1616
    Illegal PstI site found at 1816
    Illegal PstI site found at 2027
    Illegal PstI site found at 2121
    Illegal PstI site found at 2589
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1319
    Illegal XbaI site found at 7320
    Illegal PstI site found at 1616
    Illegal PstI site found at 1816
    Illegal PstI site found at 2027
    Illegal PstI site found at 2121
    Illegal PstI site found at 2589
    Illegal NgoMIV site found at 5051
    Illegal AgeI site found at 1637
    Illegal AgeI site found at 1786
    Illegal AgeI site found at 3465
    Illegal AgeI site found at 5293
    Illegal AgeI site found at 6565
    Illegal AgeI site found at 6888
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3393


Design Notes

None


Source

pACYCDuet-1, lambda genome

References