Composite

Part:BBa_K1891015:Design

Designed by: Xinyu Liu   Group: iGEM16_BNU-China   (2016-10-13)


P(3HB)(BBa_K934001)+HSP promotor (BBa_K873002)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 916
    Illegal BglII site found at 1741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 222
    Illegal NgoMIV site found at 293
    Illegal NgoMIV site found at 893
    Illegal NgoMIV site found at 1205
    Illegal NgoMIV site found at 1484
    Illegal NgoMIV site found at 2136
    Illegal NgoMIV site found at 2158
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4002


Design Notes

We used Spe I and Pst I to do dual digestion in PSB1C3 which contained HSP gene and recycled gene fragments in 2117bp, used Xbal I and Pst I to do dual digestion in PSB1C3 and recycled gene fragments in 4208bp.We use T4 ligase to link phaC1-A-B1 to the downstream of HSP promoter, then transform the vectors to Trans 5α for plasmid extraction.


Source

We get parts BBa_K934001 and BBa_K873002 from the kits sent by iGEM HQ

References