Reporter

Part:BBa_K1891013:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-11)


beta-tubulin-C- Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 832
    Illegal BamHI site found at 1615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 873
    Illegal AgeI site found at 681
    Illegal AgeI site found at 1011
    Illegal AgeI site found at 1779
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We connected this two gene fragments using TOPO clone.

Source

Beta-tubulin comes from human breast cancer cell line Mcf-7 cDNA. C- Luciferase comes from pCambia1300-C-Luciferase plasmid.

References