Part:BBa_K1887002:Design

NSR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The nsr gene can be integrated into the nisin controlled expression system to tolerate high levels of nisin concentration.

Source

Genomic DNA of L. lactis subsp. diacetylactis DRC3 was used as the template for PCR.

References

[1] Hacker, C., Christ, N.A., Duchardt-Ferner, E., Korn, S., Gobl, C., Berninger, L., et al. (2015). The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin. J Biol Chem 290, 28869-28886.

[2] Khosa, S., Lagedroste, M., and Smits, S.H. (2016). Protein Defense Systems against the Lantibiotic Nisin: Function of the Immunity Protein NisI and the Resistance Protein NSR. Front Microbiol 7, 504.

[3] Sun, Z., Zhong, J., Liang, X., Liu, J., Chen, X., and Huan, L. (2009). Novel mechanism for nisin resistance via proteolytic degradation of nisin by the nisin resistance protein NSR. Antimicrob Agents Chemother 53, 1964-1973.