Other

Part:BBa_K1887001:Design

Designed by: Yifei Lu   Group: iGEM16_TMMU_China   (2016-10-10)


His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The devices can be inserted into the His segment by the AscI restriction enzyme site.


Source

We get this part by PCR using the L. lactis genomic DNA as the template.

References

[1] Delorme, C., Ehrlich, S.D., and Renault, P. (1992). Histidine biosynthesis genes in Lactococcus lactis subsp. lactis. J Bacteriol 174, 6571-6579.

[2] Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.