Part:BBa_K1886019:Design

pSB1C3_Ho1_pcyA_Pompc_CI_GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1607
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1160
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 2899
  

Design Notes

The existence of double terminator can make it difficult for pcr or overlap pcr.

Source

The subparts are from E coli genome, obtained from iGEM team HZAU's lab.