Coding

Part:BBa_K1886004:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


sll1472 ccaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1077
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2723
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal AgeI site found at 1741
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It's not difficult to construct this part,but the strength of the promoter and the RBS will influence the abundance of protein ccaS,and finally influence the whole TCS.And there was a SpeI site and a EcoRI site,so we designed two silent mutations.The upstream of gene ccaS called sll1472 is of unknown function.



Source

Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems

References

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.