Device

Part:BBa_K1886003:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


PcpcG2-sfGFP-ccaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 269


Design Notes

We choose sfGFP as the reporter to test the green TCS. We totally designed three silent mutation to standarlize this part.



Source

From E coli genome, obtained from iGEM team HZAU's lab


References

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.