Device

Part:BBa_K1886001:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


AHL single-cycled oscillation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1970
    Illegal BsaI.rc site found at 3073


Design Notes

LAA tag has the highest degradation efficiecy in 37℃. Pay attention to the environmental temperature while using it.It can only be used in Gram-negative bacteria.



Source

From E coli genome, the subparts of this part is from iGEM Distribution kits.

References

Danino T, Mondragón-Palomino O, Tsimring L, et al. A synchronized quorum of genetic clocks[J]. Nature, 2010, 463(7279): 326-330.