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Part:BBa_K1878009:Experience

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


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UNIQ83c1cfe9f139bb8a-partinfo-00000000-QINU UNIQ83c1cfe9f139bb8a-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with SoxRS and iRFP713 to fluoresce in the presence of nitric oxide. Diethylenetriamine (DETA) was used a generator of nitric oxide (NO), since a challenge for the assay was from the fact that nitric oxide is a gas in standard conditions.

Fig 1. Results of part assay with NO induction from DETA.