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Part:BBa_K1878006:Experience

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


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UNIQ3f73aef3fe42bfb1-partinfo-00000000-QINU UNIQ3f73aef3fe42bfb1-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor in E. coli with a modified version of NarK (mNarK from 2009 UCL London's BBa_K239006) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the results of our characterization indicate that transcription is downregulated.

Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.