Composite

Part:BBa_K1878004:Experience

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1878004

User Reviews

UNIQ8c5beaf2d59293ba-partinfo-00000000-QINU UNIQ8c5beaf2d59293ba-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.

Fig 1. Results of part assay in aerobic, and anaerobic, conditions.