Composite

Part:BBa_K1878003

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


pBAD/His/iRFP713


Usage and Biology

Arabinose inducible iRFP 713 with His-tag

The original citation is as follows: The original citation is as follows: D.M. Shcherbakova and V.V. Verkhusha. "Near-infrared fluorescent proteins for multicolor in vivo imaging." Nature Methods, 2013. 10(8): p. 751-4.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1261
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1330
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1317
    Illegal BamHI site found at 1345
    Illegal XhoI site found at 1326
    Illegal XhoI site found at 1681
    Illegal XhoI site found at 1687
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1944
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2441
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None