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Part:BBa_K187422:Design

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-21)


anchor reverse strand for Biobytes assembly method


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See http://2009.igem.org/Team:Alberta/DNAanchor for binding capacity assays with the Biobytes anchor. We tested three alternative designs, which are documented on the wiki.

The anchoring system we decided on, and submitted here, is small in size and the presence of the ssDNA spacer region gives this anchor a high binding capacity of about 200 pmol mg-1 beads. The presence of the dsDNA region allows Bytes to be ligated to the anchor and the incorporation of a deoxyuracil containing I-SceI site allows the construct to be released via the two methods described already(USER and I-SceI digestion).


Source

Oligonucleotide synthesis

References