Primer

Part:BBa_K187365

Designed by: Team Biobytes   Group: iGEM09_Alberta   (2009-10-20)

pAB_F (A end) USER primer for BioBytes plasmid pAB

This is the forward primer of a pair of universal primers for amplifying any gene out of pAB (BBa_K187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.

See the BioBytes RFC for details of the BioBytes assembly method. Additional information in regards to characterization can be found at http://2009.igem.org/Team:Alberta/Optimization.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None