Composite

Part:BBa_K1871001:Design

Designed by: Broad Run High School iGEM Team   Group: iGEM15_BroadRun-NorthernVA   (2015-09-13)


Alpha amylase with native secretion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1533
    Illegal NgoMIV site found at 1548
    Illegal AgeI site found at 1851
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TBD


Source

The amylase gene was obtained from the published sequence for Bacillus amyloliquefaciens

References