Part:BBa_K187000:Experience

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Applications of BBa_K187000

This part has been tested extensively by the BioBytes team, and has worked as expected in all assays. See the Alberta wiki for more information: http://2009.igem.org/Team:Alberta. The plasmids pBA and pAB have been used in all assays for the bead assembly method, as described in the BioBytes BBF RFC.

Briefly, Biobytes is a new standardized in vitro assembly method. The system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated.

User Reviews

UNIQ448e79d3bbda7a63-partinfo-00000000-QINU UNIQ448e79d3bbda7a63-partinfo-00000001-QINU