Coding

Part:BBa_K1869001:Design

Designed by: Conary Meyer   Group: iGEM15_Santa_Clara   (2015-09-18)


SAM Synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was extracted through PCR with over hangs designed to ligate into the pSB1C3 vector using the PIPE cloning method.


Source

The SAM synthetase sequence was extracted, through PCR, from E. coli K-12 TG1. This protein is inherent in various organisms as it plays a major role in methionine and cysteine biosynthesis.

References